Evaluation of fertilization medium quality in in vitro maturation of human germinal vesicle oocytes gained during intra cytoplasmic sperm injection cycles

Background: in vitro maturation of human germinal oocytes is a promising technique in assisted reproductive technologies, different medium has been introducing in last two decays for this purpose but they are very expensive and gained by order with different qualification  Objective: the aim of study to evaluate the efficacy of fertilization medium used in in vitro maturation of germinal vesicles gained during ICSI cycles. Patients and methods: 42 oocytes in germinal vesicle stage involved in in vitro maturation (IVM) program with fertilization medium, they represent 22 cases (couples) that underwent IVF-ICSI operation, gained from them, (9) of them excluded because they are morphologically abnormal, the rest (33) GV introduced in program, they incubated for 24 hours and reassessed again to evaluate their maturation, the study done in the High institute for infertility diagnosis and assisted reproductive technologies, Al- Nahrain University from the period December 2018 to February 2020 . Results: out of (33) Gv incubation for 24 hours (6) of them remain in GV stage, (8) of them pass to M1 maturation stage, and another (8)   passes to M2 stage of maturation, the rest 11 they were atretic.&nbsp