Phytochemical analysis and GC-MS-MS investigation of methanol extract of Calotropis procera aerial parts

The purpose of our study was to examine chemical constituents of Calotropis Procera aerial parts using preliminary chemical test and GC-MS-MS analysis  since no  enough phytochemical investigation had been done regarding the plant in Iraq, the aerial parts leaves stems and fruits  of C. Procera were extracted  in 95% aqueous methanol in soxhlet  and fractionated with n-hexane , chloroform respectively . The extract was analyzed using specific chemical reactions to diagnose the chemicals specifically the important secondary metabolites, and then checked further in GC-MSMS to identify the identity of each compound