ISOLATION OF PROLACTIN BY ACRYLAMIDE GEL ELECTROPHORESIS OF THE RAT PITUITARY TISSUE CULTURE MEDIUM

Biological, chemical, or immunological properties of prolactin are not yet completely understood. The relationship between pituitary prolactin and growth hormone or prolactin-like substance in the fluids such as blood and urine in human is another important problem to study. The relationship between pituitary prolactin and growth hormone or prolactin-like substance in the fluids such as blood and urine in human is another important problem to study. In the present experiment isolation of prolactin from the rat pituitary tissue culture medium was attempted by acrylamide gel electrophoresis, utilizing the fact that the pituitary separated from hypothalamic connection secreted almost exclusively prolactin and that pituitary prolactin increased by estrogen administration. As a result of 7-day culture prolactin band was obtained separately. Presence of growth hormone band even after 7-day culture indicated existence of GIF (growth hormone-inhibiting factor) in the hypothalamus