Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status
- Muchiut, Sebastián
- Fiel, César
- Lirón, Juan Pedro
- Lloberas, María Mercedes
- Ceriani, Carolina
- Lorenzo, Ramiro
- Riva, Eliana
- Bernat, Gisele
- Cardozo, Patricia Alejandra
- Fernández, Silvina
- Steffan, Pedro
DOIAbstract
The spread of anthelmintic resistance (AR) in nematode populations threatens the viability of sheep production systems worldwide, and warrants the adoption of sensitive, practical, and standardized tests to detect AR. The aim of this study was to characterize the replacement of an Haemonchus contortus population resistant to benzimidazoles (BZDs) by a susceptible one, by means of both phenotypic and genotypic techniques. Phenotypic methods to assess BZD resistance included in vivo tests, such as the fecal egg count reduction test (FECRT), and in vitro tests, such as the egg hatch assay (EHA). Additionally, genotypification of polymorphisms associated with BZD resistance by sequencing a fragment of the isotype 1 β-tubulin gene was carried ou...
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