Bisphenol A Detection on a Carbon Graphite Electrode Modified with a Polyaniline- 2,2’-Azinobis-(3-Ethyl Benzothiazolin-6-Sulfonic Acid) Composite using Horseradish Peroxidase Enzyme as a Bio-recognition Unit, Preliminary Results

Bisphenol A is an endocrine disruptor which is almost ubiquitous. It is found in plastics, supermarket thermal receipts, CDs, food containers, water bottles, building materials amongst others. Bisphenol A has being associated with serious health issues ranging from obesity to cancer. This study aimed at developing an electrochemical method for the detection of bisphenol A in the environment. Horse radish peroxidase enzyme encapsulated on a modified polyaniline graphite carbon paste electrode was the recognition system. All electrochemical experiments were done on the cyclic voltammetric mode. The conducting properties of the polyaniline- Polyaniline 2, 2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid] polymer composites were carried out using UV-Vis spetrophotometry. The presence of polaron and bipolaron bands in the UV-Vis spectra at around 446 nm and or greater than 650 nm indicated improved polymer conductivity. Preliminary results for the HRP/H2O2 system displayed a 1.54 x10-4 peak current signal de-attenuation on addition of cumulative aliquots of 0 – 2500 nM. The greater than 90% signal de-attenuation was due to presence of BPA provided- a basis for creating a BPA detection methodology based on inhibition in future studies