Programmed cell death in the tobacco hornworm, Manduca sexta: Alteration in protein synthesis

The metamorphic death of the labial glands of the tobacco hornworm, Manduca sexta, occurs during a 4 day period during larva-to-pupa metamorphosis. The earliest changes marking the death of the cell, all occurring on the first day, are a sharp drop in protein synthesis, coupled with the selective survival or upregulation of a few messages. An early rearrangement of the rough endoplasmic reticulum is presumably related to the generalized decrease in protein synthesis. Lysosomal acid phosphatase also begins to increase very early, and ultimately the bulk of the cytoplasm is destroyed in autophagic vacuoles, but activation of lysosomes does not account for the decreased rate of synthesis. The mechanism by which most protein synthesis is depressed remains under investigation