Localization and expression characterization of ChR chimeras predicted by the models.

Measured localization and expression properties for each chimera tested and associated chimera_name, set, number of mutations, chimera_block_ID, and sequence. Chimera names and chimera_block_ID begin with either 'c' or 'n' to indicate the contiguous or non-contiguous library. The following 10 digits in the chimera_block_ID indicate, in block order, the parent that contributes each of the 10 blocks ('0':CheRiff, '1':C1C2, and '2':CsChrimR). For the contiguous library, blocks in the chimera_block_ID are listed from N- to C-termini; for the non-contiguous library the block order is arbitrary. The set for which the chimera was generated is listed. The number of mutations (m) from the closest parent for each chimera is included. Sequences list only the ChR open reading frame, the C-terminal trafficking and mKate2.5 sequences have been removed. The table shows mean properties (mKate_mean, GFP_mean, and intensity_ratio_mean) and the standard deviation of properties (mKate_std, GFP_std, and intensity_ratio_std). ND: not detected, below the limit of detection for our assay.Related Publication: Machine learning to design integral membrane channelrhodopsins for efficient eukaryotic expression and plasma membrane localization Kevin Yang Caltech PLOS Computational Biology 2017-10-23 https://doi.org/10.1371/journal.pcbi.1005786 en