Add to ORKGWe present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation
During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens as...
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly ...
Background During the past two decades, three novel coronaviruses (CoVs) have emerged to cause inter...
textabstractWe present two real-time reverse-transcription polymerase chain reaction assays for a no...
Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respirat...
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly ...
The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranea...
Since its first emergence in 2012, cases of infection with Middle East respiratory syndrome coronavi...
We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays t...
of a novel human coronavirus by real-time reverse-transcription polymerase chain reactio
Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respirat...
The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute res...
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay...
A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole co...
We developed a set of three real-time reverse transcription-polymerase chain reaction (PCR) assays t...
During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens as...
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly ...
Background During the past two decades, three novel coronaviruses (CoVs) have emerged to cause inter...
textabstractWe present two real-time reverse-transcription polymerase chain reaction assays for a no...
Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respirat...
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly ...
The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranea...
Since its first emergence in 2012, cases of infection with Middle East respiratory syndrome coronavi...
We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays t...
of a novel human coronavirus by real-time reverse-transcription polymerase chain reactio
Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respirat...
The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute res...
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay...
A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole co...
We developed a set of three real-time reverse transcription-polymerase chain reaction (PCR) assays t...
During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens as...
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly ...
Background During the past two decades, three novel coronaviruses (CoVs) have emerged to cause inter...