New Fluorene-Based Fluorescent Probe With Efficient Two-Photon Absorption

The synthesis, linear photophysical characterization, and two-photon absorption (2PA) properties of new fluorene derivative 3,3\u27-(pyridine-2,6-diyl)bis(1-(7-(diphenylamino)-9,9-dihexyl- 9H-fluoren-2-yl)propane-1,3-dione) (1) have been presented. The steady-state absorption, fluorescence and excitation anisotropy spectra along with the fluorescence decay kinetics of 1 are obtained in the solvents of different polarities at room temperature with respect to its potential application in bioimaging. The analysis of linear photophysical properties revealed a complicated nature of the main one-photon absorption band of 1, and the strong solvatochromic effect in steady-state fluorescence spectra is observed. The degenerate 2PA spectrum of 1 is measured in the spectral range 570-970 nm with the use of the open aperture Z-scan method under the 1-kHz femtosecond excitation, and the maximum values of two-photon action cross sections ~(100-130) GM are obtained. The nature of the linear absorption and the 2PA bands is analyzed by quantum chemical methods using a Gaussian program package. © M.V. BONDAR, O.V. PRZHONSKA, O.D. KACHKOVSKY, A. FRAZER, A.R. MORALES, K.D. BELFIELD, 2013