Regenerating vascular mural cells in zebrafish fin blood vessels are not derived from pre-existing ones and differentially require Pdgfrb signalling for their development

Vascular networks are comprised of endothelial cells and mural cells, which include pericytes and smooth muscle cells. To elucidate the mechanisms controlling mural cell recruitment during development and tissue regeneration, we studied zebrafish caudal fin arteries. Mural cells colonizing arteries proximal to the body wrapped around them, while those in more distal regions extended protrusions along the proximo-distal vascular axis. Both cell populations expressed platelet-derived growth factor receptor beta (pdgfrb) and the smooth muscle cell marker myosin heavy chain 11a (myh11a). Most wrapping cells in proximal locations additionally expressed acta2. Loss of Pdgfrb signalling specifically decreased mural cell numbers at the vascular front. Using lineage tracing, we demonstrate that precursor cells located in periarterial regions and expressing Pgdfrb can give rise to mural cells. Studying tissue regeneration, we did not find evidence that newly formed mural cells were derived from pre-existing ones. Together, our findings reveal conserved roles for Pdgfrb signalling in development and regeneration and suggest a limited capacity of mural cells to self-renew or contribute to other cell types during tissue regeneration.