Biliary disorders can lead to life-threatening disease and are also a challenging complication of liver transplantation. As there are limited treatment options, tissue engineered bile ducts could be employed to replace or repair damaged bile ducts. We explored how these constructs can be created by seeding hepatobiliary LGR5 + organoids onto tissue-specific scaffold. For this, we decellularized discarded human extrahepatic bile ducts (EBD) that we recellularized with organoids of different origin, that is, liver biopsies, extrahepatic bile duct biopsies, and bile samples. Here, we demonstrate efficient decellularization of EBD tissue. Recellularization of the EBD extracellular matrix (ECM) with the organoids of extrahepatic origin (EBD tiss...
Liver tissue engineering has undergone remarkable developments since the late 20th century, transiti...
Recent biotechnical advances in the in vitro culture of cholangiocytes and generation of bioengineer...
Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair o...
Biliary disorders can lead to life-threatening disease and are also a challenging complication of li...
The treatment of common bile duct (CBD) disorders, such as biliary atresia or ischemic strictures, i...
Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options whe...
The well-established 3D organoid culture method enabled efficient expansion of cholangiocyte-like ce...
Pediatric liver transplantation is often required as a consequence of biliary disorders because of t...
Pediatric liver transplantation is often required as a consequence of biliary disorders because of t...
Organoid technology holds great promise for regenerative medicine. Recent studies show feasibility f...
Design of bioengineered organs is still complicated due to the lack of understanding of biological m...
Liver tissue engineering has undergone remarkable developments since the late 20th century, transiti...
Recent biotechnical advances in the in vitro culture of cholangiocytes and generation of bioengineer...
Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair o...
Biliary disorders can lead to life-threatening disease and are also a challenging complication of li...
The treatment of common bile duct (CBD) disorders, such as biliary atresia or ischemic strictures, i...
Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options whe...
The well-established 3D organoid culture method enabled efficient expansion of cholangiocyte-like ce...
Pediatric liver transplantation is often required as a consequence of biliary disorders because of t...
Pediatric liver transplantation is often required as a consequence of biliary disorders because of t...
Organoid technology holds great promise for regenerative medicine. Recent studies show feasibility f...
Design of bioengineered organs is still complicated due to the lack of understanding of biological m...
Liver tissue engineering has undergone remarkable developments since the late 20th century, transiti...
Recent biotechnical advances in the in vitro culture of cholangiocytes and generation of bioengineer...
Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair o...