Design, Synthesis of a Novel N-Substituted Benzimidazole Derivatives and Evaluation for their Biological Activities

The synthesis of titled compounds was achieved by following synthetic routes as shown in the scheme. The synthesis of title compounds was achieved in good yield by simple techniques. It was chemically stable and available in good yield. The sharp melting point and unique spot-on TLC indicated that title compounds were obtained in pure form. The synthesized Compounds were purified by successive recrystallization from the appropriate solvents. These compounds also exhibited appropriate peaks at corresponding δ ppm in their 1H-NMR spectra and corresponding molecular ion peaks in LC-MS spectra which conformed with the assigned structures. The interpretation of IR, 1H NMR, and LC-MS spectra confirmed the structure of the title compounds. All the designed compounds were subjected to molecular docking studies using discovery studio software. All the studied compounds show the significant docking score and which is compared with standard drug Doxorubicin. All the synthesized compounds were subjected to in vitro anti-cancer activity by SRB assay using MDA-MD-231 cell line studies. Conclusion: It could be concluded from the present investigation that the benzimidazole derivative possess the most potent anticancer molecules. Future will be develop the QSAR studies and Pharmacodynamic studies perform