Formulation and Evaluation of Transdermal Patches of Eichhornia Crassipes for Wound Healing Activity

Six batches of (P1, P2, P3, S4, S5 & S6) Aqueous extract of Eichhornia crassipes transdermal patches were prepared by solvent casting technique. The various formulation parameters, Drug-Polymer ratios and permeation enhancers were optimized to get thin, transparent, smooth, stable and high permeable transdermal patches. Drug and excipient compatibility observed in 1:1 ratio, relative humidity 45℃ From the optimization, best 2 formulations P2 & S5 were selected based on physico chemical evaluation and in vitro drug diffusion study 0.3ml of glycerin was added as plasticizer to produce a flexible patch without having major influence on their diffusion property. If the amount exceeds, the film loses its flexibility and become stiff. The plasticizer diffuses through the patch and softens the polymer particles. This softening promotes latex coalescence and patch formation. All the six batches were evaluated for Percentage Moisture uptake, Percentage Moisture content, Thickness, Folding Endurance, Percentage Drug content, Percent Elongation, Tensile strength and Adhesive strength. The formulations P2 & S5 showed maximum % Moisture uptake, Moisture content, Thickness, folding endurance, % Drug content, Percent elongation, Tensile strength No significant difference in drug content was observed between the patches among the six formulations. This indicates the homogenous dispensing of drug during the patch preparation. The data obtained from in vitro diffusion profile of selected formulations were fitted with various kinetic equations to determine the mechanism of drug diffusion and diffusion rate as indicated by higher correlation coefficients (r2). The drug diffusion from P2 & S5 follows zero order and non-fickson diffusion. These findings indicates that the drug diffusion from the P2 & S5 patch wasdiffusioncontrolled. From the results of in vitro diffusion and physicochemical studies, P2 & S5 was concluded as best formulation. Then they were subjected to screening of wound healing activity.. The ex vivo studies results showed 61.01% of drug diffusion at the end of 8 h. It concluded the controlled diffusion property of through the skin The stability studies results showed that there is no significant change from its initial nature till the period of three months at 0˚C ± ˚C 7 % RH. The present work has achieved the objectives of formulation of transdermal patch of Aqueous extract of Eichhornia crassipesby using different polymers. The diffusion kinetics confirms that the formulation followed zero orde