Comparative Evaluation of Antimicrobial Efficacy of Newer Sustained Release Fillers Incorporating Cetylpyridinium Chloride (CPC) with Calcium Hydroxide and Chlorhexidine as Intracanal Medicament against E.Fecalis: An In Vitro study

AIM OF THE STUDY: To compare and evaluate the antimicrobial efficacy of Sustained Release Fillers containing CetylPyridinium Chloride, Calcium Hydroxide paste, and 2% Chlorhexidine gel as an intracanal medicament against E. faecalis. MATERIALS AND METHODS: 120 freshly extracted teeth were decoronated and root samples were standardly enlarged up to size 2 peeso reamer. Then the samples were inoculated with E.faecalis (ATCC 29212) and tested for CFU counts to ensure the inoculation of microorganisms into the root samples. The remaining 108 tooth samples were divided into the following groups - Group 1: Control Saline, Group 2: Calcium Hydroxide, Group 3: 2% Chlorhexidine, and Group 4: Sustained Release Fillers incorporating CetylPyridinium Chloride. These intracanal medicaments were placed inside the root canal and incubated under aerobic conditions. After days 1, 7, and 14, nine tooth samples were taken from each group, the medicaments were thoroughly rinsed off, and dentin debris was collected using a size 3 and 4 peeso reamer, corresponding to 200μm and 400μm respectively. Then BHI broth containing collected debris was spread onto BHI agar plates and colony-forming units were counted after incubation. RESULTS: Statistical analysis of data was done using one-way ANOVA followed by a Post hoc test for multiple pairwise comparisons. The significance value was set at P<0.05. The intergroup comparison shows that there is a highly significant difference (p