Assessment of Anti-Depressant Activity of Anisochilus Petraeus Leaves in Swiss Albino Mice

The present study has been exposed that administration of APDM could normalize behavioral, biogenic amines, monoamines, and homocysteine and neurocytokine alterations induced by swimming stress. These outcomes confirmed antidepressant-like effects of the APDM through neurochemicals, homocysteine band neurocytokine mechanisms. Ancient medicine is widely held to beneficial but generally neither the active principles nor their molecular targets are well defined, therefore, an understanding of the active components and their mechanisms of action can make such medicines more acceptable. APDM is used as an medicinal plant owing to its numerous medicinal properties. Numerous studies have shown force swims induced depression model can be used for evaluating the potential antidepressants by employing behavioural tests like FST and TST. FST and TST are commonly used behavioural despair models in rodents to predict antidepressant potential by measuring the decrease in immobility periods and both are valuable for probing the mechanism of depression. Induction of depression using force swim stress is considered as the most valid animal model of depressive behavior observed in humans after a long-term exposure to multiple stressors. In the present study, mice that was exposed to stress, exhibited greater immobility periods in the FST and TST as compared to healthy control animals, thus showed depression-like behaviour. Sub-chronic treatment with imipramine (15 mg/kg, ip) produced a significant decrease in immobility periods of stress mice in the FST and TST, indicating significant antidepressant-like activity. However, in the forced swim stress AW mice, the APDM produced a significant reduction in immobility time when orally administered for 28 consecutive days. In addition, the compounds enhancing mobility activity may give rise to a false positive effect in the stress mice. Thus, antidepressant-like activity of APDM in stressed mices is specific. APDM caused a reduction in the immobility time in FST and TST. The results presented here show, to our knowledge for the first time, that APDM given orally is effective in producing significant antidepressant-like activity, when assessed in the FST and TST. In both tests, anti-depressants can also be distinguished from stimulants, because stimulants cause marked motor stimulation, in contrast to antidepressants