Sulfated Galactans from Gracilaria Fisheri with Supplementation of Octanoyl Promote Wound Healing Activity In Vitro and In Vivo.

International audienceSulfated galactans (SG) isolated from Gracilaria fisheri was partially degraded (DSG), and subsequentially supplemented with octanoyl (DSGO) and sulfate (DSGS) groups. The molecular weights of DSG, DSGO, and DSGS were 7.87, 152.79, and 97.07\,kDa, respectively. The modification was confirmed using FTIR and NMR, whilst in vitro wound healing activity was assessed using scratched wound fibroblasts. The results revealed that DSGO exhibited highest percentage of wound closure in scratched fibroblast L929 cells. Furthermore, DSGO was able to promote proliferation and accelerate migration of scratched fibroblasts, which corresponded to the regulation of proteins and mRNA (Ki67, p-FAK, vimentin and E-cadherin) determined by Western blotting and qPCR analysis. The superior wound healing activity of DSGO was also confirmed in excision wound of rats. The results demonstrated that DSGO significantly enhanced the percentage of wound closure, re-epithelialization, and collagen arrangement, increased α-SMA and vimentin expression, and decreased that of TNF-α at the wound site. The results suggest that degraded SG supplemented with medium-chain fatty acids of octanoyl group might pass through the membrane, subsequently activating the mediators associated with proliferation and migration of fibroblasts, which could potentially lead to the promotion of wound healing activity. This article is protected by copyright. All rights reserved