Influence of poly lactic-co-glycolic acid scaffold with concentrated growth factor on human osteoblast cells

The innovation of grafting materials facilitates an improved patients’ care and treatment outcomes in promoting bone regeneration. A recent paradigm shift towards poly lactic-co-glycolic acid (PLGA) as an excellent scaffold for tissue engineering and autologous nature of concentrated growth factor (CGF) is starting to be in the highlight of treatment. However, the role of both materials as substitutes for bone grafting material remains unclear. In this study, we aimed to investigate the influence of both materials on the biological behavior of human osteoblast cells (HOBs). PLGA microspheres prepared using double solvent evaporation method were observed under scanning electron microscope. Blood was collected from a rabbit and centrifuged to obtain CGF. HOBs were incubated with CGF, PLGA microspheres, and CGF+PLGA for 24, 48, and 72 h. Their proliferation was assessed. Microscopic image revealed a spherical shape with interconnected pores as an architecture for cellular ingrowth. Significant differences observed in the mean proliferation of HOBs between control and CGF+PLGA group. Similar observations were observed between control and PLGA, and between CGF and PLGA, indicating the role of PLGA and CGF for bone regeneration. This result further indicated that both PLGA scaffold with CGF has the potential as alternative materials in promoting bone regeneration