Phase Behavior and Miscibility in Lipid Monolayers Containing Glycolipids

Hypothesis:Glycolipids in biological membranes are ubiquitous and believed to be involved in the formation of ordered functional domains. However, our current knowledge about such glycolipid-enriched domains is limited because they are inherently difficult to characterize.Experiments:We use grazing-incidence X-ray diffraction, isotherm measurements, and Brewster angle microscopy to investigate the phase behavior and miscibility in Langmuir lipid monolayers containing glycolipids.Findings:Glycolipid-enriched domains give rise to distinct diffraction patterns that allow for a systematic structural investigation and reveal a rich phenomenology, ranging from near-complete demixing to the formation of mixed domains with unique features. The phase behavior is governed by the headgroup chemistry and by the length and saturation of the tails