The thesis deals with the application of VAT on cross-border transactions. The main objective of this thesis is to analyse the impact of changes in the taxation of cross-border transactions on e-commerce in the Czech Republic, namely on the import and export of selected commodities and foreign trade with Germany and Great Britain. Data were used for both intra-community transactions and imports and exports to/from third countries. The content of the chapters is as follows. The first chapter deals with the harmonisation of VAT within the European Union. The second chapter discusses the taxation of cross-border transactions applied until 30 June 2021. The third chapter discusses changes in the taxation of cross-border transactions effective f...