A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human immunodeficiency virus type 1 (HIV-1) viremia was developed and optimized. This method consists of the reverse transcription and subsequent amplification in the same tube of two similar RNA templates, the wild-type template to be quantified and a known amount of the internally deleted synthetic template, both with identical primer recognition sites. The same strategy also proved to be useful in the quantitative assay of HIV-1-specific cellular transcripts and proviral DNA sequences from peripheral blood mononuclear cells by using competitor DNA. The method might be of interest in the study of the precise level of HIV-1 activity during the diffe...
Testing HIV-1 RNA in plasma by PCR is universally accepted as the ultimate standard to confirm diagn...
HIV-1 DNA can persist in host cells, establishing a latent reservoir. This study was aimed to develo...
The correct diagnosis andmonitoring of HIV-1 group O (HIV-O) infection are essential for appropriate...
A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human im...
An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficie...
A method for quantitating human immunodeficiency virus type 1 plasma viremia may be useful in monito...
An ultrasensitive version of an 'in-house' reverse transcription-competitive polymerase chain reacti...
An in-house reverse transcription (RT)-competitive PCR (RT-cPCR) for the quantitation of human immun...
Based on previous experience in our laboratory, we developed a real-time reverse transcriptase (RT) ...
A quantitative polymerase chain reaction (PCR) procedure has been developed for rapid retrovirus tit...
A general strategy for the construction of an internal standard for the polymerase chain reaction (P...
HIV-1 viral load represents a basic marker for evaluation of the rate and severity of HIV-1 related ...
platform) for the simultaneous quantification of total and extrachromosomal HIV-1 DNA forms in pati...
Reverse transcriptase (RT) is a crucial enzyme for retrovirus replication, and its presence in the v...
Quantification of human immunodeficiency virus type-1 (HIV-1) proviral DNA is increasingly used to m...
Testing HIV-1 RNA in plasma by PCR is universally accepted as the ultimate standard to confirm diagn...
HIV-1 DNA can persist in host cells, establishing a latent reservoir. This study was aimed to develo...
The correct diagnosis andmonitoring of HIV-1 group O (HIV-O) infection are essential for appropriate...
A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human im...
An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficie...
A method for quantitating human immunodeficiency virus type 1 plasma viremia may be useful in monito...
An ultrasensitive version of an 'in-house' reverse transcription-competitive polymerase chain reacti...
An in-house reverse transcription (RT)-competitive PCR (RT-cPCR) for the quantitation of human immun...
Based on previous experience in our laboratory, we developed a real-time reverse transcriptase (RT) ...
A quantitative polymerase chain reaction (PCR) procedure has been developed for rapid retrovirus tit...
A general strategy for the construction of an internal standard for the polymerase chain reaction (P...
HIV-1 viral load represents a basic marker for evaluation of the rate and severity of HIV-1 related ...
platform) for the simultaneous quantification of total and extrachromosomal HIV-1 DNA forms in pati...
Reverse transcriptase (RT) is a crucial enzyme for retrovirus replication, and its presence in the v...
Quantification of human immunodeficiency virus type-1 (HIV-1) proviral DNA is increasingly used to m...
Testing HIV-1 RNA in plasma by PCR is universally accepted as the ultimate standard to confirm diagn...
HIV-1 DNA can persist in host cells, establishing a latent reservoir. This study was aimed to develo...
The correct diagnosis andmonitoring of HIV-1 group O (HIV-O) infection are essential for appropriate...