Human skin penetration of gold nanoparticles through intact and damaged skin.

Gold nanoparticles (AuNPs) are produced for many applications but there is a lack of available data on their skin absorption. Experiments were performed using the Franz diffusion cell method with intact and damaged human skin. A physiological solution was used as receiving phase and 0.5 mL (1st exp) and 1.5 mL (2nd exp) of a solution containing 100 mg L\u207b\ub9 of AuNPs (15 and 45 \u3bcg cm\u207b\ub2, respectively) was applied as donor phase to the outer surface of the skin for 24 h. Skin absorption was dose dependent. Mean gold content of 214.0 \ub1 43.7 ng cm\u207b\ub2 and 187.7 \ub1 50.2 ng cm\u207b\ub2 were found in the receiving solutions of cells where the AuNPs solution was applied in higher concentration on intact skin (8 Franz cells) and on damaged skin (8 Franz cells), respectively. Twenty-four hours gold flux permeation was 7.8 \ub1 2.0 ng cm\u207b\ub2 h\u207b\ub9 and 7.1 \ub1 2.5 ng cm\u207b\ub2 h\u207b\ub9 in intact and damaged skin, respectively, with a lag time less than 1 hour. Transmission Electron Microscope analysis on skin samples and chemical analysis using Inductively Coupled Plasma-Mass Spectrometry demonstrated the presence of AuNPs into epidermis and dermis. This study showed that AuNPs are able to penetrate the human skin in an in vitro diffusion cell system