Raman and FTIR imaging of lung tissue:bronchopulmonary sequestration

Bronchopulmonary sequestration (BPS) involves abnormal pulmonary tissue lacking trancheobronchial connection and having a separate vascular supply that arises from the thoracic or abdominal aorta. For the first time, BPS is studied by Raman and Fourier-transform infrared (FTIR) imaging. Cryosections were mounted on calcium fluoride substrates from tissue samples of twoBPS patients.Ramanimages were collected at a step size of 66\u2013100 \u3bcmto assess thewhole tissue section, andat a lower step size of 10 \u3bcm to resolve details in selected areas. FTIR images were collected at resolutions of 63 and 4 \u3bcm per pixel. Data sets were segmented by cluster analyses, and the spectra of each cluster were compared. The spectra revealed higher red blood cell content and lower collagen and lipid content in BPS than in an additional tissue section from the BPS margin