Nucleus transfer in mammals:noninvasive approaches for thepreparation of cytoplastsJosef Fulka Jr1, Pasqualino Loi2, Helena Fulka1, Grazyna Ptak2 and Taku Nagai31Institute of Animal Production, POB 1, Prague 10, Czech Republic2University of Teramo, Department of Comparative Biomedical Sciences, Teramo 64100, Italy3National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, JapanThe production of cloned animals is a difficult and complexprocedure that requires two basic steps. First, thecytoplast must be prepared by the enucleation of metaphaseII oocytes. Second, the nucleus is transferredeither by fusion or by direct microinjection into the cytoplast.The preparation of cytoplasts is a crucial stepbecause they must be a...
Not AvailableThe possibility of producing interspecies handmade cloned (iHMC) embryos by nuclear tra...
Ruminants were the first mammalian species to be cloned successfully by nuclear transplantation. Tho...
Clonning studies performed in order to produce genetically idenbtical living beings are seperated in...
The transfer of nuclei from one cell to another provides a powerful tool for studying the interactio...
The establishment of human ES cells has given tremendous input to the concept of stem cell-based the...
none4siEmbryologists working with livestock species were the pioneers in the field of reprogramming ...
Cloning animals by nuclear transfer (NT) has been successful in several mammalian species. In additi...
The ability to clone mammals via somatic cell nuclear transfer has been recognized as a major scient...
nuclear transferdonor cell typesdonor ageserum starvationcell passage Cloning by nuclear transfer us...
During the natural event of fertilization highly differentiated spermatozoa may revert to the totipo...
Effective activation of a recipient oocyte and its compatibility with the nuclear donor are critical...
The present study aims to investigate the nuclear reprogramming and culture requirement for somatic ...
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. Howeve...
The introduction of cell nuclei into enucleated recipient cells (Metaphase II oocytes), besides enuc...
During the natural event of fertilization highly differentiated spermatozoa may revert to the totipo...
Not AvailableThe possibility of producing interspecies handmade cloned (iHMC) embryos by nuclear tra...
Ruminants were the first mammalian species to be cloned successfully by nuclear transplantation. Tho...
Clonning studies performed in order to produce genetically idenbtical living beings are seperated in...
The transfer of nuclei from one cell to another provides a powerful tool for studying the interactio...
The establishment of human ES cells has given tremendous input to the concept of stem cell-based the...
none4siEmbryologists working with livestock species were the pioneers in the field of reprogramming ...
Cloning animals by nuclear transfer (NT) has been successful in several mammalian species. In additi...
The ability to clone mammals via somatic cell nuclear transfer has been recognized as a major scient...
nuclear transferdonor cell typesdonor ageserum starvationcell passage Cloning by nuclear transfer us...
During the natural event of fertilization highly differentiated spermatozoa may revert to the totipo...
Effective activation of a recipient oocyte and its compatibility with the nuclear donor are critical...
The present study aims to investigate the nuclear reprogramming and culture requirement for somatic ...
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. Howeve...
The introduction of cell nuclei into enucleated recipient cells (Metaphase II oocytes), besides enuc...
During the natural event of fertilization highly differentiated spermatozoa may revert to the totipo...
Not AvailableThe possibility of producing interspecies handmade cloned (iHMC) embryos by nuclear tra...
Ruminants were the first mammalian species to be cloned successfully by nuclear transplantation. Tho...
Clonning studies performed in order to produce genetically idenbtical living beings are seperated in...