FLOW-THROUGH ANALYSIS OF GLUTATHIONE IN HUMAN ERYTHROCYTES WITH AN AMPEROMETRIC BIOSENSOR

A flow-through system for the determination of glutathione was developed using the enzyme glutathione oxidase covalently immobilized on an Immobilon AV membrane assembled on a Clark type oxygen electrode. A calibration curve for glutathione, was obtained in the range 10(-5) - 10(-3) mol/L with a detection limit of 5 . 10(-6) mol/L and a relative standard deviation of 4.5%.[...