Feeding Frequency Differently Affects Post Prandial Patterns of Plasma Glucose, Insulin and Insulin-like Growth Factor I in European Sea Bass (Dicentrarchus labrax)

The aim of this study was to investigate the effects of feeding frequency on post-prandial pattern of circulating glucose, insulin and IGF-I (insulin-like growth factor-I) in European sea bass (D. labrax). Fish (average body weight 72.1 \ub1 2 g) were randomly allocated in 12 tanks, reared in a closed thermo regulated recirculating system and fed a commercial diet at a fixed ration (1.5% body weight) for 7 weeks. Six fish groups were fed the ration in one meal a day (08:30 am), while six fish groups were offered the same ration in two daily meals (08:30 am and 04:30 pm) according to a complete random design. Plasma insulin mean level was affected by the splitting up of the ration (14.4 vs 15.2 ng/ml, P