Optimization of Oxygen Radical Absorbance Capacity method for antioxidant activity of extracts from oregano (lat.Origanum vulgare)

Listovi origana (lat. Origanum vulgare) dobar su izvor fenolnih spojeva (antioksidansa) s velikom mogućnošću primjene u farmaceutskoj i prehrambenoj industriji. Cilj ovog eksperimenta bio je efikasno provesti ekstrakciju suhih listova origana uz pomoć visokonaponskog električnog pražnjenja (HVED metode). Dobiveni rezultati uspoređeni su s efikasnošću ekstrakcije konvencionalnom metodom. Tretman HVED-om provodio se s različitim vremenom tretiranja, različitim otapalima, različitim radnim plinovima i pri različitim naponima. Kod dobivenih vrijednosti može se uočiti djelomična serija pravilnosti. Prilikom povećanja volumnog udjela etanola, vremena tretiranja i napona dolazi do povećanja vrijednosti antioksidacijskog kapaciteta. Najveći prinos bio je kod uzoraka koji su tretirani 9 min, pri 20/25 kV i u 50 %-tnoj otopini etanola.Oregano leaves (lat. Origanum vulgare) are useful source of phenolic compounds (antioxidants) with great possibility of application in Pharmaceutical and Food industry. The goal of this experiment was to efficiently carry out an extraction of dried oregano leaves using high voltage electric discharge (HVED method). The results were compared with the extraction efficiency by conventional method. The HVED treatment was performed with different treatment times, different solvents, different working gases and at different voltages. Sample values shows a partial series of regularities. With a rise in ethanol concentration, time and voltage treatment, an increase in antioxidant capacity is observed. The greatest yield was observed in samples which were treated with argon for 9 min, at 20/25 kV and in a 50% ethanol solution