Serological diagnostics of cytomegalovirus

Cilj rada: Svrha rada je pojasniti način serološke dijagnostike citomegalovirusa u Nastavnom zavodu za javno zdravstvo Splitsko-dalmatinske županije. Materijali i metode: Postupak serološke dijagnostike CMV-a je određivanje IgM i IgG protutijela. Prisutnost IgM antitijela ukazuje na akutnu infekciju, a IgG na prošlu infekciju. U dijagnostičku svrhu određivanja CMV-a u Nastavnom zavodu za javno zdravstvo Splitsko-dalmatinske županije koriste se kitovi za određivanje protutijela Abbott Alinity i. Sam analizator je Abbott Alinity i. Metoda kojom određuje protutijela je CMIA. CMIA je skraćeni naziv za kemiluminiscentnu imunokemijsku metodu. Uzorak je serum koji se izdvaja centrifugiranjem krvi. Serum se stavlja u stalak i potom u aparat koji analizira prisutnost protutijela. Rezultati: Serološkim ispitivanjem uzorka pacijenta određena je prisutnost IgG proutijela CMV-a, a odsutnost IgM što ukazuje na preboljenu citomegalovirusnu infekciju.Objectives: The purpose of this bachelor's thesis is to clarify the method of serological diagnosis of cytomegalovirus at the Teaching Institute for Public health of Split-Dalmatia County. Materials and methods: The procedure of serological diagnosis of CMV includes the detection of IgM and IgG antibodies. The presence of IgM antibodies indicates acute infection while IgG indicates past infection. For the diagnostic purpose, at the Teaching Institute of Public Health of Split-Dalmatia County, kits Abbott Alinity i are used to detect CMV antibody. The analyzer is also called Abbott Alinity i. The method used to determine antibodies is CMIA. CMIA is the abbreviation for the chemiluminescent immunochemical method. The sample is a serum that is extracted by centrifugation of blood. The serum is placed in a rack and then in an analyzer that analyzes the presence of antibodies. Results: Serological examination of the patient's sample determined the presence of IgG antibodies to CMV, and the absence of IgM, which indicates a past cytomegalovirus infectio