The coat proteins of the RNA bacteriophages Qβ and MS2 are specific RNA binding proteins. Although they possess common tertiary structures, they bind different RNA stem loops and thus provide useful models of specific protein-RNA recognition. Although the RNA-binding site of MS2 coat protein has been extensively characterized previously, little is known about Qβ. Here we describe the isolation of mutants that define the RNA-binding site of Qβ coat protein, showing that, as with MS2, it resides on the surface of a large β-sheet. Mutations are also described that convert Qβ coat protein to the RNA binding specificity of MS2. The results of these and other studies indicate that, although they bind different RNAs, the binding sites of the two c...
Using a combination of biochemistry, mass spectrometry, NMR spectroscopy and cryo-electron microscop...
114 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.The importance of RNA as a me...
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also...
The coat proteins of the RNA bacteriophages Qβ and MS2 are specific RNA binding proteins. Although t...
The coat proteins of the RNA phages MS2 and Qβ are structurally homologous, yet they specifically bi...
The coat proteins of the RNA phages MS2 and Qβ are structurally homologous, yet they specifically bi...
SummarySequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vit...
The coat proteins of RNA phages MS2 and GA are specific RNA-binding proteins which function to encap...
The coat proteins of different single-strand RNA phages use a common protein tertiary structural fra...
We present the results of In vitro binding studies aimed at defining the key recognition elements on...
In MS2 assembly of phage particles results from an interaction between a coat protein dimer and a st...
AbstractBackground: The coat protein in RNA bacteriophages binds and encapsidates viral RNA, and als...
The icosahedrally symmetrized structure of bacteriophage MS2 as determined by cryo-electron microsco...
114 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.The importance of RNA as a me...
145 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997.The coat protein from bacteri...
Using a combination of biochemistry, mass spectrometry, NMR spectroscopy and cryo-electron microscop...
114 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.The importance of RNA as a me...
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also...
The coat proteins of the RNA bacteriophages Qβ and MS2 are specific RNA binding proteins. Although t...
The coat proteins of the RNA phages MS2 and Qβ are structurally homologous, yet they specifically bi...
The coat proteins of the RNA phages MS2 and Qβ are structurally homologous, yet they specifically bi...
SummarySequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vit...
The coat proteins of RNA phages MS2 and GA are specific RNA-binding proteins which function to encap...
The coat proteins of different single-strand RNA phages use a common protein tertiary structural fra...
We present the results of In vitro binding studies aimed at defining the key recognition elements on...
In MS2 assembly of phage particles results from an interaction between a coat protein dimer and a st...
AbstractBackground: The coat protein in RNA bacteriophages binds and encapsidates viral RNA, and als...
The icosahedrally symmetrized structure of bacteriophage MS2 as determined by cryo-electron microsco...
114 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.The importance of RNA as a me...
145 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997.The coat protein from bacteri...
Using a combination of biochemistry, mass spectrometry, NMR spectroscopy and cryo-electron microscop...
114 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.The importance of RNA as a me...
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also...