Eukaryotic Initiation Factor 4E (eIF4E) in Complex With eIF4E Binding Protein 1 (4E-BP1) Binds With Higher Affinity to m7GpppN Cap of a Subset of Human mRNAs

Fluorescence anisotropy binding assays were used to analyze the binding of eIF4E in complex with 4E-BP1 onto the 5’ m7G cap of a subset of mRNA that are known to carry cap-independent translation. These studies suggest that 4E-BP1 increases eIF4E binding affinity to 5’cap of both FGF-9 and HIF-1