A Study of the Effect of Supplemented Growth Regulators on Phalaenopsis Amabilis Micropropagation Using Temporary Immersion System

This research studies the effect of supplemented growth regulators on Phalaenopsis amabilis micropropagation using temporary immersion system (TIS). The experiment results show that MS supplemented with BA 2 mg/l, NAA 1 mg/l, DCC 30 g/l, peptone 1 g/l, sucrose 30 g/l, CW 20%, CA 1 g/l is the most appropriate culture medium for PLBs. MS½ supplemented with BA 2 mg/l, DCC 30 g/l, Peptones 1 g/l, sucrose 30 g/l, CW 20%, CA 0.5 g/l is the best culture medium for shoot regeneration. MS½ supplemented with IBA 2 mg/l, DCC 30 g/l, Peptones 1 g/l, sucrose 30 g/l, CW 20%, CA 1 g/l is the best culture medium for developing roots. The rates of PLBs regeneration and shoot multiplication on TIS are 2-3 times higher than those on agar culture medium