Extraction of Lipase From Papaya Latex and an Examinination of Its Characteristics

Lipase from plants, which possesses various interesting features, could be utilized for application in different fields. In this study, the crude lipase is obtained from the insoluble fraction of papaya latex. Some biochemical properties of this lipase are studied by two methods: titrimetric and photometric one. The results show that the catalytic activity of the lipase from papaya latex is high and optimized at the temperature 45 0C and the pH 8.5. The presence of Na+, Ca2+, Mg2+, Mn2+, Cu2+, EDTA decreases the activity of lipase. However, the inhibition degree of Ca2+and Mg2+ is much higher than Na+ and EDTA. This research also aims at successfully releasing lipase out of “its natural rubber covering”. This lipase hydrolyzes fish oil at much larger activity than olive oil