Biodegradation of Malachite Degradation by Laccsae From Bacillus Licheniformis NS2324

Malachite green, a triphenylmethane dye, is used in aquaculture to limit the growth of protozoans and fungi in fish. It is also employed in the food, medicinal, and textile sectors. MG has high toxicity covering micro organisms and higher eukaryotes. Its toxic effects include organ damage, developmental abnormalities and mutagenic/carcinogenic potentials. Hence it is of utmost importance to degrade MG. Therefore, in this study with the help of catalytic properties of laccase, biodegradation of dye-malachite green was studied with respect to different factors like temperature, pH, enzyme dose and treatment time. The maximum of 99.31±0.97%  MG decoloration was obtained under the conditions of 10 U ml-1 enzyme, pH 8.0 incubation time 1h and a temperature of 50°C. MG was degraded without any mediator of laccase in the current study