Callus Inducement and Strawberry Plant Regeneration From Leaves

Strawberry seeds (Fragaria vescaL.) are processed at 4°C for 5 days and are germinated on MS medium for 6 weeks. In this study, the strawberry leaves tissue is used to induce callus in the dark for 10 days and 20 days light on MS medium supplemented with 0.2 mg/l (2,4-D) and 1.0 mg/l (TDZ), after 3 – 4 weeks of callus appearance with 85.3 percent. The callus regenerates on MS medium supplemented with 0.2 mg/l of BAP and 1.0 mg/l. of TDZ. The callus regenerates buds well with 91.7 percent and 60.6 percent at the concentrations of 0.2 mg/l of BAP combined with 1.0 mg/l of TDZ and 0.2 mg/l of Kinetin combined with 1.0 mg/l ofTDZ respectively. Shoots multiply with 10.2 buds and 4.38 in the two formulations above, after 10 weeks. Strawberries have high roots on MS medium supplemented with 0.5 mg/l of IBA.The average of roots is 92.8 percent, averaging 3- 5 roots/plant. The length of roots is from 0.8 to 3.2 cm. The results indicate that breeding strawberries through callus results in high regeneration and propagation coefficient