Phytochemical Analysis and Antioxidant Effect of Hexane Extract of Cassia Fistula Using FT-IR and GC-MS Analysis

The aim of the present investigation is screening of phytochemical constituents for its free radical scavenging activity of hexane extract of Cassia fistula leaves. The preliminary screening was carried out by standard chemical method. It evidenced that the presence of phytochemicals such as alkaloids, anthraquinones, saponins, phenols, tannins, flavonoids and terpenoids in the hexane extract. Various functional groups like alcohols, phenols, carboxylic acid and ketones were associated with the extract were characterized using Fourier transform infrared spectroscopy (FTIR). A few common compounds available in the extract were identified by Gas chromatography – Mass spectrum (GC-MS) analysis. The DPPH free radical scavenging activity and 50 % inhibitory concentration (IC50) was calculated and compared with the standard synthetic drug BHT. This study revealed that C. fistula hexane extract of leaves has alternative drugs for free radical it could be acted as a novel source of free radical scavengers