Ultra Performance Liquid Chromatography: A New Revolution in Liquid Chromatography

Ultra Performance Liquid Chromatography (UPLC) can be regarded as a new direction for liquidchromatography. UPLC improves three areas of Liquid chromatography namely speed, resolution, and sensitivity of analysis by using particle size less than 2 ?m and the system is designed in a special way to withstand high system back-pressures. The separation on UPLC is performed under very high pressures (up to 100 MPa) but it has no negative influence on analytical column or other components of chromatographic system and also decrease of time and solvent consumption compare to HPLC. Now a day's pharmaceutical industries as well as analytical laboratories are in search of new ways to reduce cost and time for analysis ofdrugs and improve quality of their product. This review focus on basic principle, instrumentation and theory of UPLC. It also summarizes various pharmaceutical applications of this technique with examples