The Role of Increased Matrix Metalloproteinase (MMP14) Expression on Apoptosis Sensitivity in Human Breast Cancer Cells

A member of the matrix metalloproteinase family (MMP14) plays a crucial role in extracellular matrix (ECM) remodeling, as it possesses characteristics that aid in cell proliferation and differentiation. MMP14 domains can bind to FIH-1, which is a protein that can prevent apoptosis in cells when attached to BAX in the mitochondria. This study will investigate the varying expressions of MMP14 on FIH-1 and BAX, and their effects on apoptotic sensitivity. Three cell lines with varying levels of MMP14 expression will be used: C3 (~11 fold), ΔCD (~100 fold), and C2 (~500 fold). With the overexpression of MM14 on the cell surface, cells are more susceptible to death, as FIH-1 has a higher affinity for the MMP14 domain as opposed to BAX. Caspase-Glo 3/7 Assay system will be used to assess apoptotic sensitivity after Staurosporine treatments. In essence, the significance of this research may aid in new therapeutic strategies for cancer treatments