Comparison of hydrolysis and HPLC/MS/MS procedure with ELISA assay for the determination of S-phenylmercapturic acid as a biomarker of benzene exposure in human urine

TRANFO G
PIGINI D
PACI E
DORIA D
MANNO M
BARTOLUCCI, GIOVANNI BATTISTA
SCAPELLATO, MARIA LUISA
CARRIERI, MARIELLA

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Publication date

January 2010

DOI

10.1016/j.jchromb.2009.11.011

ISSN

1570-0232

Citation count (estimate)

Abstract

Abstract The present study compared three methods for the determination of S-phenylmercapturic acid (S-PMA), a metabolite of benzene, in human urine: a HPLC/MS/MS technique with two different sample treatments (strong and partial hydrolysis) and a commercial assay based on anti-S-PMA monoclonal antibodies with chemiluminescence detection. Biological monitoring was done on 126 volunteers and the results were compared for the three methods and also with benzene exposure levels (range <3.0-592.5 μg/m(3)). The correlation between environmental monitoring data and S-PMA levels in non-smokers (n=73) was highly significant (p<0.0001, Student's t-test) for both HPLC/MS/MS methods (r=0.65 both for strong acidic hydrolysis of the urine and hydrolysi...