In this chapter, we review the theoretical and experimental foundations underling a quantitative approach to Ca2+ imaging, discuss equilibrium conditions and their violations and present a computational framework that can be used to estimate the spatial and temporal dynamics of Ca2+ signals based of fluorescence measurements with Ca2+ indicators
Cytosolic Ca2+ ions regulate numerous aspects of cellular activity in almost all cell types, control...
We extend the sensitivity of quantitative concentration imaging to an approximately 1000-fold range ...
Ca2+ ions are vital for several functions in the human body such as muscle contraction, neuronal com...
Imaging Ca2+-sensitive fluorescent indicators provides a common approach for studying Ca2+ signals i...
AbstractCalcium-sensitive dual excitation dyes, such as fura-2, are now widely used to measure the f...
Viability of cells is strongly related to their Ca2+ homeostasis. Ca2+ signal fluctuations can be on...
AbstractLocal intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual c...
In many situations, fluorescent Ca2+ reporters are used to simply indicate that a change of Ca2+ con...
International audienceCa(2+) imaging is a commonly used approach for measuring Ca(2+) signals at hig...
International audienceMeasuring variations of intracellular free calcium concentration through the c...
Abstract- We describe the use of a new imaging technology, fluorescence lifetime imaging (FLIM), for...
Cellular Ca2+ signals are often constrained to cytosolic micro- or nano-domains where stochastic ope...
<p>(A) Calcium fluorescence signal (dots) together with the least-squares fit of the calcium excitat...
Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels ...
This paper is a short review of the fluorimetric methods used to measure intracellular free Ca++ con...
Cytosolic Ca2+ ions regulate numerous aspects of cellular activity in almost all cell types, control...
We extend the sensitivity of quantitative concentration imaging to an approximately 1000-fold range ...
Ca2+ ions are vital for several functions in the human body such as muscle contraction, neuronal com...
Imaging Ca2+-sensitive fluorescent indicators provides a common approach for studying Ca2+ signals i...
AbstractCalcium-sensitive dual excitation dyes, such as fura-2, are now widely used to measure the f...
Viability of cells is strongly related to their Ca2+ homeostasis. Ca2+ signal fluctuations can be on...
AbstractLocal intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual c...
In many situations, fluorescent Ca2+ reporters are used to simply indicate that a change of Ca2+ con...
International audienceCa(2+) imaging is a commonly used approach for measuring Ca(2+) signals at hig...
International audienceMeasuring variations of intracellular free calcium concentration through the c...
Abstract- We describe the use of a new imaging technology, fluorescence lifetime imaging (FLIM), for...
Cellular Ca2+ signals are often constrained to cytosolic micro- or nano-domains where stochastic ope...
<p>(A) Calcium fluorescence signal (dots) together with the least-squares fit of the calcium excitat...
Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels ...
This paper is a short review of the fluorimetric methods used to measure intracellular free Ca++ con...
Cytosolic Ca2+ ions regulate numerous aspects of cellular activity in almost all cell types, control...
We extend the sensitivity of quantitative concentration imaging to an approximately 1000-fold range ...
Ca2+ ions are vital for several functions in the human body such as muscle contraction, neuronal com...