Avaliar o efeito do receptor P2X 7 no sistema renina- angiotensina durante a progressão da nefropatia diabética

Aim: To analyze the effect of P2X7 receptor on RAS in DN. Methods: Uninephectomized male Wistar rats received a single dose of streptozotocin (60 mg/kg, i.v) for diabetes mellitus (DM) induction; control animals (CTL) received the drug vehicle. The animals were submitted to P2X7 receptor silencing with single puncture directly in the kidneys, forming the group (DM+siRNA). The animals were placed in metabolic cages before and after the protocol, for data collection and evaluation of renal function; at the end of the protocol, the animals were sacrificed and the kidney was removed for protein content analysis of P2X7R, renin, angiotensin- converting enzyme (ACE) and ACE2. RAS enzyme activity, thiobarbituric acid reactive substance levels (TBARS) and nitric oxide (NO) were analyzed; qualitative histological analysis was also performed on renal tissue. Data were expressed as mean ± standard error, with significance for p