Assessing stress response in lizards from agroecosystems with different management practices

Despite the importance of reptiles in agroecosystems, little is known about the effects of agricultural intensification and pesticide use on these animals. We compared antioxidant and haematological biomarkers in the wild Italian wall lizards Podarcis siculus from three olive groves representing a gradient of management intensity. Lizards from the conventional grove showed induced antioxidant defences relative to those from the organic field. However, this induction did not avoid the occurrence of oxidative stress in males from intensively managed olive groves, who showed TBARS levels 58%–133% higher than males from the other sites. Haematological responses also suggested increased stress in females from the intensively managed olive groves, with a heterophil-to-lymphocyte ratio 5.3 to 14.8-fold higher than in the other sites. The observed stress responses of lizards along the studied gradient of agricultural management suggest their potential usefulness as non-destructive biomarkers to environmental stressors associated with agricultural intensification.The present study was supported by FEDER through COMPETE – Programa Operacional Factores de Competitividade, Fundação para a Ciência e Tecnologia (PTDC/BIA-BIC/3488/2012), CESAM’s Strategic Programme (UIDB/50017/2020 + UIDP/50017/2020), the EU’s FP7 Project HERPESTI (IEF-2012-328328) and the Doctoral Fellowship of EF (SFRH/BD/88955/2012).Peer reviewe