HMF uptake and transport from added culture medium and breakfast cereals in Caco-2 cells

Resumen del póster presentado al COST Action 927-IMARS Joint Workshop, celebrado en Napoles del 24 al 27 de mayo de 2006.Hydroxymethylfurfural (HMF), a common intermediate in the Maillard reaction also formed by sugar degradation, has been detected in many foods. lts toxicological relevance is stlll uncertain, since in vitro studies on genotoxicity/mutagenicity have given controversial results. A previous step to analyse the impact of HMF consumption on health may be the study of its uptake/transport in the Caco-2 cell line, as a modal to predict the in vivo avaliability. The aim of this work was to test the HMF uptake/transport from HMF added culture medium and from in vitro digested breakfast cereals. 1 )The culture medium was added with three different levels of HMF until final concentrations of 130, 65 and 13 mgiL HMF (DMEM-1, -2 and -3, respectively). 2) HMF was analysed in three different breakfast cereals (A, B and C), before and after the in vitro digestion (in the supernatants obtained). 3) Caco-2 cells were seaded into bicameral chambers (Transwell). On day 21 post-seeding, test solutions (DMEM-1, -2, -3 and supernatants of A, B and C digestad samples) wera added to the apical chambers; a transport solutlon, HMF free, placed in the basolateral chamber and cell cultures adaquately incubated for 2h. After incubationHMF transported across the cell monolayer and that internalised into the cells durlng the experiment was measured by HPLC. After the in vitro digestion, only a 13.2, 56.4 and 46.5% of the HMF presant in A, B and C samples was soluble. The uptake and the transport of HMF by the cells were demonstrated in the enriched culture medium and in the digested breakfast cereals. In tha last ones, the initial concentration of HMF was not dlrectly related to the soluble fraction after digestion neither to the %HMF transported, showing, as expected, an effect of tha sample composition in the availabillty of HMF.Peer reviewe