Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases

The feasibility of biochemical transformation processes is usually greatly dependent on biocatalysts cost. Therefore, immobilizing and reusing biocatalysts is an approach to be considered to bring biotransformations closer to industrial feasibility, since it does not only allow to reuse enzymes but can also improve their stability towards several reaction conditions. Carbohydrate-Binding Modules (CBM) are well-described domains involved in substrate binding which have been already used as purification tags.This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under Grant Agreement n°761042 (BIOCON-CO2). This output reflects the views only of the author and the European Comission cannot be held responsible for any use which may be made of the information contained therein. The research group is recognized by Generalitat de Catalunya as 2017 SGR 1462.Peer reviewe