Carbon remineralization by small mesopelagic and bathypelagic Stomiiforms in the Northeast Atlantic Ocean

14 pages 11 figures, 3 tables, supplementary data https://doi.org/10.1016/j.pocean.2022.102787The organic carbon resulting from photosynthesis in the upper ocean is transferred downward through the passive sinking of organic particles, physical mixing of particulate and dissolved organic carbon as well as active flux transported by zooplanktonic and micronektonic migrants. Several meso- and bathypelagic organisms feed in shallower layers during the nighttime and respire, defecate, excrete and die at depth. Recent studies suggest that migrant micronekton transport similar amounts of carbon to migrant zooplankton. However, there is scarce information about biomass and carbon flux by non-migratory species in the mesopelagic and bathypelagic zones. The non-migratory bristlemouth fishes (Cyclothone spp.) and partial migrator (A. hemigymnus) remineralise organic carbon at depth, and knowledge about this process by this fauna is lacking despite them having been referred to as the most abundant vertebrates on Earth. Here we show the vertical distribution of biomass and respiration of non-migratory mesopelagic fishes, during day and night, using the enzymatic activity of the electron transfer system (ETS) as a proxy for respiration rates. The study is focused on five Cyclothone species (C. braueri, C. pseudopallida, C. pallida, C. livida and C. microdon) and Argyropelecus hemigymnus. The samples were taken on a transect from the oceanic upwelling off Northwest Africa (20° N, 20° W) to the south of Iceland (60° N, 20° W). Cyclothone spp. showed, by far, the largest biomass (126.90 ± 86.20 mg C·m−2) compared to A. hemigymnus (0.54 ± 0.44 mg C·m−2). The highest concentrations of Cyclothone spp. in the water column were observed between 400 and 600 m and from 1000 to 1500 m depths, both during day and night. For the different species analysed, ETS activity did not show significant differences between diurnal and nocturnal periods. The total average specific respiration of Cyclothone spp. (0.02 ± 0.01 d-1) was lower than that observed for A. hemigymnus (0.05 ± 0.02 d-1). The average carbon respiration of Cyclothone spp. was 2.22 ± 0.81 mg C·m−2·d-1, while it was much lower for A. hemigymnus (0.04 ± 0.03 mg C·m−2·d-1). The respiration of Cyclothone spp. was lower in the bathypelagic than in the mesopelagic zone (0.84 ± 0.48 vs 1.36 ± 1.01 mg C·m−2·d-1, respectively). These results, to our knowledge, provide the first account of remineralisation by this community in the meso and bathypelagic zones of the oceanThis work was funded by the Spanish Government through the project Bathypelagic (CTM2016-78853-R), and by the European Union (Horizon 2020 Research and Innovation Programme) through projects SUMMER (Grant Agreement 817806), TRIATLAS (Grant Agreement 817578) and the Spanish Government through the Project Desafío (PID2020-118118RB-I00). Airam Sarmiento-Lezcano was supported by a postgraduate grant (BES-2017-082540) and José María Landeira by the “Beatriz Galindo” grant (BEAGAL 18/ 00172) both from the Spanish Ministry of Science and Innovation. Laia Armengol was funded with a postdoctoral fellowship from “Unidad de Océano y Clima” of R&D&I Consejo Superior de Investigaciones Científicas and IOCAG, and a postdoctoral fellowship from project South and Tropical Atlantic Climate-Based Marine Ecosystem Prediction for Sustainable Management (TRIATLAS, 817578; European Commission) and Ione Medina-Suárez by project MAC-CLIMA (MAC2/3.5b/254) from the European Union. MPO acknowledges the institutional support of the AEI ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewe