Novel competition test for food rewards reveals stable dominance status in adult male rats

Social hierarchy is a potent modulator of behavior, that is typically established through overt agonistic interactions between individuals in the group. Once established, social ranks are maintained through subtler interactions allowing the redirection of energy away from agonistic interactions towards other needs. The available tasks for assessing social rank in rats allow the study of the mechanisms by which social hierarches are formed in early phases but fail to assess the maintenance of established hierarchies between stable pairs of animals, which might rely on distinct neurobiological mechanisms. Here we present and validate a novel trial-based dominancy assay, the modified Food Competition test, where established social hierarchies can be identified in the home cage of non-food deprived pairs of male rats. In this task, we introduce a small conflict in the home cage, where access to a new feeder containing palatable pellets can only be gained by one animal at a time. We found that this subtle conflict triggered asymmetric social interactions and resulted in higher consumption of food by one of the animals in the pair, which reliably predicted hierarchy in other tests. Our findings reveal stable dominance status in pair-housed rats and provide a novel tool for the evaluation of established social hierarchies, the modified Food Competition test, that is robust and easy to implement.This work was supported by grants of the NARSAD Young Investigator Grant from the Brain & Behavior Research Foundation under the grant number 26478 to C.M., the Spanish Agency of Research (grant RTI2018-097843-B-100 to C.M.), the “Severo Ochoa” Program for Centers of Excellence in R&D (SEV-2013-0317 and SEV-2017-0723) and the Champalimaud Foundation. D.F.C. was further supported by the Ministerio de Ciencia e Innovación (BES-2016-07674) and C.M. by a Ramon y Cajal contract (RYC-2014-16450).Peer reviewe