High Fat Diet effect in the tumor development of a glioblastoma mouse model

Trabajo presentado en el 1st ISMRM Iberian Chapter Annual Meeting, celebrado en Portugal del 16 al 17 de junio de 2021.Obesity is a severe disease characterized by accumulation of fat that has grown to epidemic proportions, with over 4 million people dying every year. It induces important alterations in liver, pancreas or brain, and is associated with other diseases like diabetes or cancer (1). However, the influence of overweight in brain tumours is still unclear. On these grounds, an orthotopic glioblastoma (GBM) mouse model was developed in mice fed with standard diet (SD), and mice submitted to high-fat diet (HFD) and investigated the influence of diet in GBM magnetic resonance characterization (2). Two cohorts (n=12) of C57BL6/J male adult mice with HFD and SD feeding were used. Animals were submitted to GL261 glioma cells intracranial injection 10 weeks after diet diversification. MRI studies were performed 21-24 days after. Anatomical images and T2, T2*, MT and DTI maps were acquired in a 7T system. Parametric maps were generated by adjusting the signal to the corresponding equation. Four regions of interest were manually selected: i)tumor core, ii) tumor periphery, iii) peritumoral region and iv) contralateral area. Statistical analysis was performed by two-way ANOVA tests, with area and diet as factors, and post-hoc multiple T-test comparisons with Bonferroni corrections. Data obtained from MRI maps pointed to higher MD, FA, Dax, T2* and MTR values in obese mice, while Drad and T2 were higher for control cohort. The results are consistent with a higher inflammatory state in the brain of obese GBM animals compared to control, agreeing previous results obtained in mice without cancer (3)