Exploration of sterilization method and type of media for in vitro propagation of bauhinia scandens

This study aims to obtain an appropriate sterilization method and media for propagation of Bauhinia scandens in vitro. The study was conducted at the Tissue Culture Laboratory and Biology Greenhouse. The method used in this study was induction using cytokinin BAP (benzyl amino purine) in MS media. Explain obtained from seed seeds that have been germinated in polybags with soil media and compost. Sterilization for planting with tissue culture techniques using detergents, 70% alcohol, 20% and 10% Clorox, and sterile aqua dest. The media used were MS (Murashige and Skoog) media plus BAP (concentrations of 2 and 4 ppm), as well as 2,4-D (0.4, 1.0, and 1.5 ppm) and the tones, were added with activated charcoal. The results showed that the addition of BAP in MS media was able to trigger the growth of shoots in explorer node bauhinia at concentrations of 2 and 4 ppm. The addition of 2,4-D was able to induce callus in Bauhinia scandens leaves. At a concentration of 1 ppm the callus produced was white, runny with leaves that were not too curled while concentrations of 0.4 and 1.5 ppm produced green callus with curled leaves. More varied media treatments need to be performed on both nodes and leaf explants to produce plantlets through callus or shoot formation directly