Municipal Wastewater: A Sustainable Source for the Green Microalgae Chlorella vulgaris Biomass Production

The need to reduce the costs associated with microalgae cultivation encouraged scientific research into coupling this process with wastewater treatment. Thus, the aim of this work was to assess the growth of Chlorella vulgaris (Chlorophyta) in different effluents from a municipal wastewater treatment plant (WWTP), namely secondary effluent (SE) and sludge run-off (SR). Assays were performed, under the same conditions, in triplicate with 4 dilution ratios of the wastewaters (25%, 50%, 75% and 100%) with the standard culture medium bold basal medium double nitrated (BBM2N) as a control. The capability of C. vulgaris for biomass production, chlorophyll synthesis and nutrients removal in the SE and SR was evaluated. The 25% SE and 25% SR showed increased specific growth rates (0.47 and 0.55 day1, respectively) and higher biomass yields (8.64 107 and 1.95 107 cells/mL, respectively). Regarding the chlorophyll content, the 100% SR promoted the highest concentration of this pigment (2378 g/L). This green microalga was also able to remove 94.8% of total phosphorus of SE, while in 50% SR, 31.2% was removed. Removal of 73.9% and 65.9% of total nitrogen in 50% and 100% SR, respectively, was also observed. C. vulgaris growth can, therefore, be maximized with the addition of municipal effluents, to optimize biomass production, while cleansing the effluents