Expression level of DEK isoforms.

(A) RT-PCR results show endogenous mRNA expression levels of each DEK isoform in human cell lines. The same amount of RNA and cDNA was analyzed for each sample by using primers specific to either DEK1 or DEK2 mRNA. GAPDH analysis was performed for the normalization. (B) RT-qPCR analyses of the same cell lines that were shown in the upper panel. HepG2 cells were used as a calibrator in these analyses. DEK1 and DEK2 expression that was normalized with GAPDH (left and middle panel) or DEK2 expression that was normalized with DEK1 (right panel) were shown. (C) The expression level of DEK1 and DEK2 in HS-27A cell lines that overexpress Flag (left panel) or Myc (right panel) tagged DEK isoforms was analyzed by RT-qPCR. The expression level of each isoform was normalized with GAPDH expression. Fold change was calculated by using the expression level of the DEK1 isoform in the control cells (GFP-only) as a calibrator. "Target" indicates the primer pairs that were specific to each isoform, recognizing both ectopic and endogenous mRNAs. (D) Western blot analyses show ectopic expression of Flag (left panel) or Myc (right panel) epitope-tagged DEK1 and DEK2 in HS-27A cells. Anti-GAPDH antibody was used to show equal loading.