Combination treatment reduces cell viability and induces apoptosis in BV-173 cells.

(A) Ph+ cells BV-173 were seeded with DMSO, dasatinib 50nM, bortezomib 6.25nM and the combination at day 0, drugs were replenished every third day, respectively. Viability was measured every 24h until day 5 using PI staining. (B) At day 3 cell viability was significantly reduced to only 7.27%±6.6% with bortezomib treatment and 7.16±4.8% with the combination treatment whereas the dasatinib only treated cells were 78.62±4.5% viable. For all analyses, p values were calculated by one-way ANOVA with Bonferroni multiple comparison test. A p value of less than 0.05 was considered statistically significant for all analyses. (*,p<0.05, **,p<0.01, ***,p<0.001), error bars = SD, n = 3/3).