Tumor cell line-educated moDCs polarize the T cells in different ways.

CD14+ monocytes were cultured with 100 ng/ml IL-4, 80 ng/ml GM-CSF ± MDA-MB231-, HeLa-, HT29-, WM278-, WM1617-, WM983A- or WM983B-CM for four days. Control or TU-CM-moDCs were co-cultured with autologous peripheral blood lymphocytes (PBL) for three, five, or nine days in RPMI-1640 medium at a ratio of 1:10 (moDCs: T-cells). The cytokine production capacity of T cells was measured by flow cytometry. The figure shows the mean plus SD of the populations positive for the measured surface molecules and cytokines calculated from at least six/four independent experiments. Histograms or dot plots show one of the six/four independent experiments. Significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001.